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Activation of spleen tyrosine kinase is required for TNF-α-induced endothelin-1 upregulation in human aortic endothelial cells
06 February 2012
Yoon Ji Kim, Tai Yeon Koo, Won Seok Yang, Nam Jeong Han, Jin Uk Jeong, Sang Koo Lee, Su-Kil Park
Highlights: ► TNF-α-induced ROS activates Syk and PI3K. ► These are required to activate AP-1 and subsequent ET-1. ► ET-1 is implicated in the pathogenesis of atherosclerosis. ► Syk could be a possibl...
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Asp563 of the horizontal helix of subunit NuoL is involved in proton translocation by the respiratory complex I
06 February 2012
Stefan Steimle, Max Willistein, Patricia Hegger, Marco Janoschke, Heiko Erhardt, Thorsten Friedrich
Highlights: ► The respiratory complex I contains an unusual, horizontal’ helix on subunit NuoL. ► We mutated residues of the helix to investigate their role in proton translocation. ► The variants D56...
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Two sides of the story? Smad4 loss in pancreatic cancer versus head-and-neck cancer
06 February 2012
Stephen P. Malkoski, Xiao-Jing Wang
Abstract: TGFβ signaling Smads (Smad2, 3, and 4) were suspected tumor suppressors soon after their discovery. Nearly two decades of research confirmed this role and revealed other divergent and cancer...
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Insights into differential modulation of receptor function by hinge region using novel agonistic lutropin receptor and inverse agonistic thyrotropin receptor antibodies
03 February 2012
Ritankar Majumdar, Reema Railkar, Rajan R Dighe
Highlights: ► First report of novel agonistic and antagonistic scFvs against LHR Hinge region. ► Discontinuous epitope of agonistic scFv comprises regions in both Cb-2 and Cb-3. ► Development of inver...
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Construction of synthetic regulatory networks in yeast
03 February 2012
Benjamin A. Blount, Tim Weenink, Tom Ellis
Abstract: Yeast species such as Saccharomyces cerevisiae have been exploited by humans for millennia and so it is therefore unsurprising that they are attractive cells to re-engineer for industrial us...
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